il 17 inhibitor y 320 Search Results


y 320  (MedChemExpress)
94
MedChemExpress y 320
Y 320, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+17+inhibitor+y+320/Y-320/pm38493816-71-13-18
Average 94 stars, based on 1 article reviews
y 320 - by Bioz Stars, 2026-09
94/100 stars
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il 17 inhibitor y320  (Selleck Chemicals)
90
Selleck Chemicals il 17 inhibitor y320
MALT1 expression is elevated upon PRRSV infection. (A and B) Marc-145 cells were infected with PRRSV HuN4 (MOI, 1) for the indicated time periods. (A) MALT1 mRNA and protein expression was detected in qRT-PCR and Western blot, respectively. (B) PRRSV N protein and MALT1 were immunostained with anti-PRRSV N MAb VH13 (green) and anti-MALT1 pAb (red), respectively. Nuclei were labeled with DAPI (blue). (C) Marc-145 cells were transfected with plasmids expressing different nsps or pCDNA3.1 empty vector (EV). mRNA levels of MALT1 were detected by qRT-PCR 24 h after transfection. (D) Marc-145 cells were transfected with nsp4, -7β, or -11 plasmids or EV. MALT1 protein levels were detected in Western blot analysis. (E) Marc-145 cells were cotransfected with His-nsp and MALT1 plasmids. nsp and MALT1 signaling was visualized using anti-His MAb (green) and anti-MALT1 MAb (red), respectively. (F) Marc-145 cells were incubated with different concentrations of IL-17 (10 to 500 ng/mL) for 12 h (left) or with 200 ng/mL of IL-17 for different time periods (right). MALT1 levels were detected in Western blot analysis. (G) Marc-145 cells were incubated with IL-17 inhibitor <t>Y320</t> at different concentrations (0 to 300 nM) for 24 h and subsequently transfected with pCDNA-nsp11 or empty vector. Culture supernatant was replaced with complete medium containing Y320 at indicated concentrations at 6 h posttransfection. Cells were collected 24 h after transfection for detection of MALT1 expression in Western blot analysis.
Il 17 Inhibitor Y320, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+17+inhibitor+y+320/Y-320/pmc09239189-275-14-17
Average 90 stars, based on 1 article reviews
il 17 inhibitor y320 - by Bioz Stars, 2026-09
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pu 1580 hplc  (JASCO Inc)
98
JASCO Inc pu 1580 hplc
MALT1 expression is elevated upon PRRSV infection. (A and B) Marc-145 cells were infected with PRRSV HuN4 (MOI, 1) for the indicated time periods. (A) MALT1 mRNA and protein expression was detected in qRT-PCR and Western blot, respectively. (B) PRRSV N protein and MALT1 were immunostained with anti-PRRSV N MAb VH13 (green) and anti-MALT1 pAb (red), respectively. Nuclei were labeled with DAPI (blue). (C) Marc-145 cells were transfected with plasmids expressing different nsps or pCDNA3.1 empty vector (EV). mRNA levels of MALT1 were detected by qRT-PCR 24 h after transfection. (D) Marc-145 cells were transfected with nsp4, -7β, or -11 plasmids or EV. MALT1 protein levels were detected in Western blot analysis. (E) Marc-145 cells were cotransfected with His-nsp and MALT1 plasmids. nsp and MALT1 signaling was visualized using anti-His MAb (green) and anti-MALT1 MAb (red), respectively. (F) Marc-145 cells were incubated with different concentrations of IL-17 (10 to 500 ng/mL) for 12 h (left) or with 200 ng/mL of IL-17 for different time periods (right). MALT1 levels were detected in Western blot analysis. (G) Marc-145 cells were incubated with IL-17 inhibitor <t>Y320</t> at different concentrations (0 to 300 nM) for 24 h and subsequently transfected with pCDNA-nsp11 or empty vector. Culture supernatant was replaced with complete medium containing Y320 at indicated concentrations at 6 h posttransfection. Cells were collected 24 h after transfection for detection of MALT1 expression in Western blot analysis.
Pu 1580 Hplc, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+17+inhibitor+y+320/JASCO+UV+Visible+PDA+Detectors/pmc04366505-115-11-10
Average 98 stars, based on 1 article reviews
pu 1580 hplc - by Bioz Stars, 2026-09
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bio plex pro human cytokine 27 plex assay  (Bio-Rad)
92
Bio-Rad bio plex pro human cytokine 27 plex assay
MALT1 expression is elevated upon PRRSV infection. (A and B) Marc-145 cells were infected with PRRSV HuN4 (MOI, 1) for the indicated time periods. (A) MALT1 mRNA and protein expression was detected in qRT-PCR and Western blot, respectively. (B) PRRSV N protein and MALT1 were immunostained with anti-PRRSV N MAb VH13 (green) and anti-MALT1 pAb (red), respectively. Nuclei were labeled with DAPI (blue). (C) Marc-145 cells were transfected with plasmids expressing different nsps or pCDNA3.1 empty vector (EV). mRNA levels of MALT1 were detected by qRT-PCR 24 h after transfection. (D) Marc-145 cells were transfected with nsp4, -7β, or -11 plasmids or EV. MALT1 protein levels were detected in Western blot analysis. (E) Marc-145 cells were cotransfected with His-nsp and MALT1 plasmids. nsp and MALT1 signaling was visualized using anti-His MAb (green) and anti-MALT1 MAb (red), respectively. (F) Marc-145 cells were incubated with different concentrations of IL-17 (10 to 500 ng/mL) for 12 h (left) or with 200 ng/mL of IL-17 for different time periods (right). MALT1 levels were detected in Western blot analysis. (G) Marc-145 cells were incubated with IL-17 inhibitor <t>Y320</t> at different concentrations (0 to 300 nM) for 24 h and subsequently transfected with pCDNA-nsp11 or empty vector. Culture supernatant was replaced with complete medium containing Y320 at indicated concentrations at 6 h posttransfection. Cells were collected 24 h after transfection for detection of MALT1 expression in Western blot analysis.
Bio Plex Pro Human Cytokine 27 Plex Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+17+inhibitor+y+320/Lyphochek+Unassayed+Chemistry+Control+(Human)/ppr0288801-80-9-43
Average 92 stars, based on 1 article reviews
bio plex pro human cytokine 27 plex assay - by Bioz Stars, 2026-09
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preparative hplc  (JASCO Inc)
99
JASCO Inc preparative hplc
MALT1 expression is elevated upon PRRSV infection. (A and B) Marc-145 cells were infected with PRRSV HuN4 (MOI, 1) for the indicated time periods. (A) MALT1 mRNA and protein expression was detected in qRT-PCR and Western blot, respectively. (B) PRRSV N protein and MALT1 were immunostained with anti-PRRSV N MAb VH13 (green) and anti-MALT1 pAb (red), respectively. Nuclei were labeled with DAPI (blue). (C) Marc-145 cells were transfected with plasmids expressing different nsps or pCDNA3.1 empty vector (EV). mRNA levels of MALT1 were detected by qRT-PCR 24 h after transfection. (D) Marc-145 cells were transfected with nsp4, -7β, or -11 plasmids or EV. MALT1 protein levels were detected in Western blot analysis. (E) Marc-145 cells were cotransfected with His-nsp and MALT1 plasmids. nsp and MALT1 signaling was visualized using anti-His MAb (green) and anti-MALT1 MAb (red), respectively. (F) Marc-145 cells were incubated with different concentrations of IL-17 (10 to 500 ng/mL) for 12 h (left) or with 200 ng/mL of IL-17 for different time periods (right). MALT1 levels were detected in Western blot analysis. (G) Marc-145 cells were incubated with IL-17 inhibitor <t>Y320</t> at different concentrations (0 to 300 nM) for 24 h and subsequently transfected with pCDNA-nsp11 or empty vector. Culture supernatant was replaced with complete medium containing Y320 at indicated concentrations at 6 h posttransfection. Cells were collected 24 h after transfection for detection of MALT1 expression in Western blot analysis.
Preparative Hplc, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+17+inhibitor+y+320/JASCO+HPLC/10__1016_slash_j__ejmech__2020__112754-356-7-6
Average 99 stars, based on 1 article reviews
preparative hplc - by Bioz Stars, 2026-09
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MALT1 expression is elevated upon PRRSV infection. (A and B) Marc-145 cells were infected with PRRSV HuN4 (MOI, 1) for the indicated time periods. (A) MALT1 mRNA and protein expression was detected in qRT-PCR and Western blot, respectively. (B) PRRSV N protein and MALT1 were immunostained with anti-PRRSV N MAb VH13 (green) and anti-MALT1 pAb (red), respectively. Nuclei were labeled with DAPI (blue). (C) Marc-145 cells were transfected with plasmids expressing different nsps or pCDNA3.1 empty vector (EV). mRNA levels of MALT1 were detected by qRT-PCR 24 h after transfection. (D) Marc-145 cells were transfected with nsp4, -7β, or -11 plasmids or EV. MALT1 protein levels were detected in Western blot analysis. (E) Marc-145 cells were cotransfected with His-nsp and MALT1 plasmids. nsp and MALT1 signaling was visualized using anti-His MAb (green) and anti-MALT1 MAb (red), respectively. (F) Marc-145 cells were incubated with different concentrations of IL-17 (10 to 500 ng/mL) for 12 h (left) or with 200 ng/mL of IL-17 for different time periods (right). MALT1 levels were detected in Western blot analysis. (G) Marc-145 cells were incubated with IL-17 inhibitor Y320 at different concentrations (0 to 300 nM) for 24 h and subsequently transfected with pCDNA-nsp11 or empty vector. Culture supernatant was replaced with complete medium containing Y320 at indicated concentrations at 6 h posttransfection. Cells were collected 24 h after transfection for detection of MALT1 expression in Western blot analysis.

Journal: mBio

Article Title: Porcine Reproductive and Respiratory Syndrome Virus Adapts Antiviral Innate Immunity via Manipulating MALT1

doi: 10.1128/mbio.00664-22

Figure Lengend Snippet: MALT1 expression is elevated upon PRRSV infection. (A and B) Marc-145 cells were infected with PRRSV HuN4 (MOI, 1) for the indicated time periods. (A) MALT1 mRNA and protein expression was detected in qRT-PCR and Western blot, respectively. (B) PRRSV N protein and MALT1 were immunostained with anti-PRRSV N MAb VH13 (green) and anti-MALT1 pAb (red), respectively. Nuclei were labeled with DAPI (blue). (C) Marc-145 cells were transfected with plasmids expressing different nsps or pCDNA3.1 empty vector (EV). mRNA levels of MALT1 were detected by qRT-PCR 24 h after transfection. (D) Marc-145 cells were transfected with nsp4, -7β, or -11 plasmids or EV. MALT1 protein levels were detected in Western blot analysis. (E) Marc-145 cells were cotransfected with His-nsp and MALT1 plasmids. nsp and MALT1 signaling was visualized using anti-His MAb (green) and anti-MALT1 MAb (red), respectively. (F) Marc-145 cells were incubated with different concentrations of IL-17 (10 to 500 ng/mL) for 12 h (left) or with 200 ng/mL of IL-17 for different time periods (right). MALT1 levels were detected in Western blot analysis. (G) Marc-145 cells were incubated with IL-17 inhibitor Y320 at different concentrations (0 to 300 nM) for 24 h and subsequently transfected with pCDNA-nsp11 or empty vector. Culture supernatant was replaced with complete medium containing Y320 at indicated concentrations at 6 h posttransfection. Cells were collected 24 h after transfection for detection of MALT1 expression in Western blot analysis.

Article Snippet: LPS and IL-17 (absin, Shanghai, China), MALT1 inhibitor Mi-2, lysosome inhibitor chloroquine (CQ) and IL-17 inhibitor Y320 (Selleck, China), and proteasome inhibitor MG-132 (Beyotime) were used at the designated concentrations, prepared in dimethyl sulfoxide (DMSO) (Sigma) or water according to the manufacturers’ instructions.

Techniques: Expressing, Infection, Quantitative RT-PCR, Western Blot, Labeling, Transfection, Plasmid Preparation, Incubation